An Undergraduate Experiment Using Cyclodextrin – Assisted Sensitive Fluorescence Detection and Quantitation of Dapsone Drug in Wastewater Samples

The guest-host interaction between Dapsone drug and β-cyclodextrin (β-CD) was investigated using fluorescence spectroscopy, 1H-NMR, and liquid chromatography with fluorescence detection. The optimized conditions for the interaction were investigated by spectrofluorometry and were found to be at 0.2 mg/mL (0.176 mM) of β-CD and pH 8.8. For these conditions, very low concentration of Dapsone drug of 2.4 ng/mL (12.97 nM) can be detected. The standard addition method was utilized to detect Dapsone in influent and effluent wastewater samples in the sub parts per billion concentration range by HPLC-FLD using β-CD as an additive in the mobile phase