The Expression of Immunomodulation-Related Cytokines and Genes of Adipose- and Bone Marrow-Derived Human Mesenchymal Stromal Cells from Early to Late Passages

Comparative immunophenotypes and cellular properties of AD-MSCs and BM-MSCs through cell passages. A, B MSCs were stained with surface antibodies and analyzed by flow cytometry. AD-MSCs and BM-MSCs were negative for CD14, CD31, CD34, CD45, CD106, and positive for CD29, CD44, CD73, CD90, and CD105. In AD-MSCs, CD29 expression was slightly increased in P10 compared to AD-MSC P4, but this was not statistically significant. In BM-MSCs, the expressions of CD90 and CD105 were slightly decreased in P10 compared to the P4. But, this was not statistically significant. C Photographs of AD- and BM-MSCs showed spindle-shaped fibroblastic morphological features in early (P4) and late passages (P10). Scale bar = 40 μm. D Doubling times for AD- and BM-MSCs were slightly delayed after repeated passages. Doubling time was determined during each sub cultivation. All values are mean？±？SE of the mean (SEM