%0 Journal Article
%T The Expression of Immunomodulation-Related Cytokines and Genes of Adipose- and Bone Marrow-Derived Human Mesenchymal Stromal Cells from Early to Late Passages
%A Chin Hee Mun
%A Mi-Il Kang
%A Yeseul Kim
%A Yong Dae Shin
%A Yong-Beom Park
%J Archive of "Tissue Engineering and Regenerative Medicine".
%D 2018
%R 10.1007/s13770-018-0147-5
%X Comparative immunophenotypes and cellular properties of AD-MSCs and BM-MSCs through cell passages. A, B MSCs were stained with surface antibodies and analyzed by flow cytometry. AD-MSCs and BM-MSCs were negative for CD14, CD31, CD34, CD45, CD106, and positive for CD29, CD44, CD73, CD90, and CD105. In AD-MSCs, CD29 expression was slightly increased in P10 compared to AD-MSC P4, but this was not statistically significant. In BM-MSCs, the expressions of CD90 and CD105 were slightly decreased in P10 compared to the P4. But, this was not statistically significant. C Photographs of AD- and BM-MSCs showed spindle-shaped fibroblastic morphological features in early (P4) and late passages (P10). Scale bar = 40 ¦̀m. D Doubling times for AD- and BM-MSCs were slightly delayed after repeated passages. Doubling time was determined during each sub cultivation. All values are mean£¿¡À£¿SE of the mean (SEM
%K Cytokine
%K Gene expression
%K Immune modulation
%K Mesenchymal stromal cells
%K Passage
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250650/