Immunoglobulin G modulation of the melanocortin 4 receptor signaling in obesity and eating disorders

a Dissociation equilibrium constants (KD). b Association rates (ka). c Dissociation rates (kd). d–h Representative sensorgrams illustrating different affinity kinetics of IgG binding to α-MSH in surface plasmon resonance units (RU) from each study group: d Ctrl (n？=？65), e obese (n？=？17), f anorexia nervosa (n？=？28), g bulimia nervosa (n？=？34), and h binge eating disorder (n？=？14). IgG concentrations, 3360？nM (in pink), 1680 nM (in red), 840？nM (in blue), 420？nM (in grey), 210？nM (in green). Fitting with the Langmuir’s 1:1 model. i Relative to controls plasma levels of α-MSH-reactive IgG after their extraction from plasma. Data are means？±？s.e.m. Kruskal–Wallis test with Dunns’ post-tests, ***p？<？0.001, **p？<？0.01, *p？<？0.05 (b, c, i) or analysis of variance with Tukey’s post-test, $$$p？<？0.001 vs. Ctrl (i