Clozapine-dependent inhibition of EGF/neuregulin receptor (ErbB) kinases

Treatment with 0.1% DMSO (vehicle control), 30？μM olanzapine (OLZ), 30？μM clozapine (CLZ), 30？μM haloperidol (HAL), or 30？μM risperidone (RIS) for 48？h affected the proliferation/survival of U87MG cells (a) and A431 cells (b) grown in the normal growth medium. The number of living cells was estimated using the CCK-8 cell counting kit before and after the drug treatment. The graph reveals % ratio to the final cell number of control cultures treated with DMSO alone (mean？±？SE, n？=？6 sister cultures). Brown–Forsythe test assessed the homogeneity of data variance in a (P？=？0.227), but not in b (P？=？0.075). One-way ANOVA followed by Tukey’s test was applied to a; **P？<？0.01 and ***P？<？0.001 vs. control culture (DMSO). β？<？0.001 and η2？=？0.799 for a. Multiple comparisons of Welch t-test with Bonferroni’s compensation was performed with the given difference in data variance among groups in b; **P？<？0.01/4 and ***P？<？0.001/4 vs. control culture (DMSO). β？=？0.923, 0.000, 0.000, and 0.600; d？=？0.307, ？4.662, ？6.799, and ？1.090 for OLZ, CLZ, HAL, and RIS vs. DMSO, respectivel