Fatostatin induces pro- and anti-apoptotic lipid accumulation in breast cancer

a ER+ (MCF-7 and T47D) and ER？ (MDA-MB-231 and BT20) breast cancer cells were treated with FS at doses indicated and cell confluency was measured over 7 days. Media was changed and cells were retreated every 2–3 days. b The IC50 (concentration of FS leading to 50% inhibition of the cell growth) was determined based on confluency on day 7. c MCF-7 cells were treated with FS for 48？h and cell cycle analysis was carried out using a BrdU assay. d–f MCF-7 cells were treated with 5？μM FS for 48？h and cell viability was measured (d). Apoptosis was measured using caspase 3/7 substrate cleavage (e) and Alexa Fluor 488 Annexin V staining (f). * P？<？0.05 vs none; ** P？<？0.01 vs non