%0 Journal Article
%T Fatostatin induces pro- and anti-apoptotic lipid accumulation in breast cancer
%A G. Ekin Atilla-Gokcumen
%A Isin T. Sakallioglu
%A Jeanne M. Danes
%A Jonna Frasor
%A Lauren M. Morrow
%A Oleskii Dubrovskyi
%A Viktor Brovkovych
%A Yasir Izhar
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0076-0
%X a ER+ (MCF-7 and T47D) and ER£¿ (MDA-MB-231 and BT20) breast cancer cells were treated with FS at doses indicated and cell confluency was measured over 7 days. Media was changed and cells were retreated every 2¨C3 days. b The IC50 (concentration of FS leading to 50% inhibition of the cell growth) was determined based on confluency on day 7. c MCF-7 cells were treated with FS for 48£¿h and cell cycle analysis was carried out using a BrdU assay. d¨Cf MCF-7 cells were treated with 5£¿¦ÌM FS for 48£¿h and cell viability was measured (d). Apoptosis was measured using caspase 3/7 substrate cleavage (e) and Alexa Fluor 488 Annexin V staining (f). * P£¿<£¿0.05 vs none; ** P£¿<£¿0.01 vs non
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107643/