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- 2017
Impact of enzymatic digestion on bacterial community composition in CF airway samplesDOI: 10.7717/peerj.3362 Keywords: 16s Sequencing, Microbiome, Sputum, Gram positive, Beta diversity, DNA extraction Abstract: Previous studies have demonstrated the importance of DNA extraction methods for molecular detection of Staphylococcus, an important bacterial group in cystic fibrosis (CF). We sought to evaluate the effect of enzymatic digestion (EnzD) prior to DNA extraction on bacterial communities identified in sputum and oropharyngeal swab (OP) samples from patients with CF
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