%0 Journal Article
%T Impact of enzymatic digestion on bacterial community composition in CF airway samples
%A Brandie D. Wagner
%A Charles E. Robertson
%A Edith T. Zemanick
%A Emily J. Johnson
%A J. Kirk Harris
%A Kayla M. Williamson
%J Archive of "PeerJ".
%D 2017
%R 10.7717/peerj.3362
%X Previous studies have demonstrated the importance of DNA extraction methods for molecular detection of Staphylococcus, an important bacterial group in cystic fibrosis (CF). We sought to evaluate the effect of enzymatic digestion (EnzD) prior to DNA extraction on bacterial communities identified in sputum and oropharyngeal swab (OP) samples from patients with CF
%K 16s Sequencing
%K Microbiome
%K Sputum
%K Gram positive
%K Beta diversity
%K DNA extraction
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452939/