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OALib Journal期刊
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-  2017 

An improved butanol-HCl assay for quantification of water-soluble, acetone:methanol-soluble, and insoluble proanthocyanidins (condensed tannins)

DOI: 10.1186/s13007-017-0213-3

Keywords: Tannin, Proanthocyanidins, Leaf litter, Nitrogen stress, Polyphenol, Flavonoid, Poplar, Douglas-fir

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Abstract:

Flow diagram of solvent and reagent concentrations during assay preparation. Solutions are listed in the order in which they are added for assaying total-soluble, water-soluble or insoluble (or total) condensed tannins. Respective volumes used are also listed to clarify changes in concentrations. All listed ratios represent v/v and concentrated HCl (~12 N). The iron reagent was 2% w/v NH4Fe(SO4)2 and H2O in 2 N HC

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