%0 Journal Article %T An improved butanol-HCl assay for quantification of water-soluble, acetone:methanol-soluble, and insoluble proanthocyanidins (condensed tannins) %A C. Peter Constabel %A J. A. Trofymow %A Philip-Edouard Shay %J Archive of "Plant Methods". %D 2017 %R 10.1186/s13007-017-0213-3 %X Flow diagram of solvent and reagent concentrations during assay preparation. Solutions are listed in the order in which they are added for assaying total-soluble, water-soluble or insoluble (or total) condensed tannins. Respective volumes used are also listed to clarify changes in concentrations. All listed ratios represent v/v and concentrated HCl (~12 N). The iron reagent was 2% w/v NH4Fe(SO4)2 and H2O in 2 N HC %K Tannin %K Proanthocyanidins %K Leaf litter %K Nitrogen stress %K Polyphenol %K Flavonoid %K Poplar %K Douglas-fir %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539752/