NF1 deficiency correlates with estrogen receptor signaling and diminished survival in breast cancer

In-frame and premature indels are present in Nf1 G0 rats. a CRISPR–Cas9 targeting of the Nf1 GRD domain in exon 20 using two sgRNAs (red). PAM sequences (green) and the Hpy81 site (purple) used for HMA/restriction digest analysis are shown. b Heteroduplex PCR amplicons from 19 Nf1 G0 animals. c DNA and peptide sequence alignment of the in-frame deletions within Nf1 exon 20. d Heteroduplex PCR amplicons from 19 Nf1 G0 animals were digested with Hpy81 to reveal the presence of smaller indels. No change between the ？Hpy81 and +Hpy81 indicates the Hpy81 restriction digestion was lost during CRISPR recombination events. e DNA and peptide sequence alignment of the small indels at the 5′ and 3′ CRISPR target regio