%0 Journal Article
%T NF1 deficiency correlates with estrogen receptor signaling and diminished survival in breast cancer
%A Anil K. Challa
%A Ashley N. Turner
%A Bryn Eagleson
%A Carrie R. Graveel
%A Curt J. Essenburg
%A Elizabeth A. Tovar
%A Eve E. Gardner
%A Matthew R. Steensma
%A Megan E. Callaghan
%A Megan J. Bowman
%A Patrick S. Dischinger
%A Robert A. Kesterson
%A Roderick T. Bronson
%A Tristan Kempston
%A Zachary B. Madaj
%J Archive of "NPJ Breast Cancer".
%D 2018
%R 10.1038/s41523-018-0080-8
%X In-frame and premature indels are present in Nf1 G0 rats. a CRISPR¨CCas9 targeting of the Nf1 GRD domain in exon 20 using two sgRNAs (red). PAM sequences (green) and the Hpy81 site (purple) used for HMA/restriction digest analysis are shown. b Heteroduplex PCR amplicons from 19 Nf1 G0 animals. c DNA and peptide sequence alignment of the in-frame deletions within Nf1 exon 20. d Heteroduplex PCR amplicons from 19 Nf1 G0 animals were digested with Hpy81 to reveal the presence of smaller indels. No change between the £¿Hpy81 and +Hpy81 indicates the Hpy81 restriction digestion was lost during CRISPR recombination events. e DNA and peptide sequence alignment of the small indels at the 5¡ä and 3¡ä CRISPR target regio
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117327/