MiR-221/222 promote epithelial-mesenchymal transition by targeting Notch3 in breast cancer cell lines

miR-221/222 have an inverse correlation with Notch3 in breast cancer cells. a qRT-PCR analysis of Notch3 mRNA expression in breast cancer cell lines. b Endogenous Notch3 full length (FL) and the intracellular domain (ICD), ERα, E-cadherin, and Vimentin protein levels in breast cancer cell lines. c, d Databases to predict miR-221/222 target genes and sequence alignment of the human miR-221 and miR-222 seed regions with Notch3 3′UTR, which contains predicted miR-221/222 binding site. e qRT-PCR analysis of miR-221/222 expression in breast cancer cell line