LRIG2 is a growth suppressor of Hec-1A and Ishikawa endometrial adenocarcinoma cells by regulating PI3K/AKT- and EGFR-mediated apoptosis and cell-cycle

a Hec-1A and Ishikawa cells were transfected with plasmids encoding LRIG2–GFP (50 or 100？ng) or siRNA #1 (100 or 200？nM) against LRIG2. As controls, either an empty vector or scrambled siRNAs were transfected. Cell viability was measured 24？h after transfection. b LRIG2–GFP-overexpressing Hec-1A cells were analyzed to detect the annexin-V-positive apoptotic cells by flow cytometry. Representative scatter plots (top) and quantified results (bottom) are shown. c Cell lysates of LRIG2–GFP-overexpressing Hec-1A cells were subjected to immunoblotting for caspases using the indicated antibodies. d Subcellular fractionation was performed using Hec-1A cells after LRIG2–GFP transfection. Cytosolic release of cytochrome c was determined by western blotting. Efficient fractionation was confirmed by immunoblotting of β-actin and COX IV. All quantified results are mean？±？SEM of three independent experiments performed in triplicates (*p？<？0.05