%0 Journal Article
%T LRIG2 is a growth suppressor of Hec-1A and Ishikawa endometrial adenocarcinoma cells by regulating PI3K/AKT- and EGFR-mediated apoptosis and cell-cycle
%A Dae-Shik Suh
%A Hanyong Jin
%A Jeehyeon Bae
%A Kangseok Lee
%A Si Eun Park
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-017-0019-1
%X a Hec-1A and Ishikawa cells were transfected with plasmids encoding LRIG2¨CGFP (50 or 100£¿ng) or siRNA #1 (100 or 200£¿nM) against LRIG2. As controls, either an empty vector or scrambled siRNAs were transfected. Cell viability was measured 24£¿h after transfection. b LRIG2¨CGFP-overexpressing Hec-1A cells were analyzed to detect the annexin-V-positive apoptotic cells by flow cytometry. Representative scatter plots (top) and quantified results (bottom) are shown. c Cell lysates of LRIG2¨CGFP-overexpressing Hec-1A cells were subjected to immunoblotting for caspases using the indicated antibodies. d Subcellular fractionation was performed using Hec-1A cells after LRIG2¨CGFP transfection. Cytosolic release of cytochrome c was determined by western blotting. Efficient fractionation was confirmed by immunoblotting of ¦Â-actin and COX IV. All quantified results are mean£¿¡À£¿SEM of three independent experiments performed in triplicates (*p£¿<£¿0.05
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833696/