LXRα limits TGFβ-dependent hepatocellular carcinoma associated fibroblast differentiation

Human HCC cells were cultured and the expression of several genes and proteins was assessed. a mRNA expression was assessed via real-time PCR and normalized to the expression of GAPDH. Mean？±？SD values are plotted. Experiments were performed in biological triplicates (nb？=？3), each of them in technical triplicate (nt？=？3). b–g The indicated human HCC cells were stimulated with DMSO (Mock), 5？ng/ml TGFβ1, 5？μM T0901317 or a combination of the last two for 72？h prior to protein immunoblot. The β-actin immunoblot serves as loading control for all panels (b–g). Experiments were performed in biological triplicates (nb？=？3). Densitometric values of LXRa protein are also included. Molecular size markers are indicated in kDa. Graph bars and immunoblot images are color-marked: epithelial (red), HepG2-mixed phenotype (blue), mesenchymal (green