%0 Journal Article
%T LXR¦Á limits TGF¦Â-dependent hepatocellular carcinoma associated fibroblast differentiation
%A Anita Mor¨¦n
%A Aristidis Moustakas
%A Carl-Henrik Heldin
%A Claudia Bellomo
%A Dimitris Kardassis
%A Wolfgang Mikulits
%A Yutaro Tsubakihara
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0140-4
%X Human HCC cells were cultured and the expression of several genes and proteins was assessed. a mRNA expression was assessed via real-time PCR and normalized to the expression of GAPDH. Mean£¿¡À£¿SD values are plotted. Experiments were performed in biological triplicates (nb£¿=£¿3), each of them in technical triplicate (nt£¿=£¿3). b¨Cg The indicated human HCC cells were stimulated with DMSO (Mock), 5£¿ng/ml TGF¦Â1, 5£¿¦ÌM T0901317 or a combination of the last two for 72£¿h prior to protein immunoblot. The ¦Â-actin immunoblot serves as loading control for all panels (b¨Cg). Experiments were performed in biological triplicates (nb£¿=£¿3). Densitometric values of LXRa protein are also included. Molecular size markers are indicated in kDa. Graph bars and immunoblot images are color-marked: epithelial (red), HepG2-mixed phenotype (blue), mesenchymal (green
%K Cancer
%K Cell biology
%K Molecular biology
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522550/