Chromosomal instability-induced senescence potentiates cell non-autonomous tumourigenic effects

a, b Schematic representation of aneuploidy induction using either (a) 8 or 16？h nocodazole (Noc) or (b) 0.2 or 1？μM reversine (Rev). c Representative images of metaphase spreads from RPE-1 shp53 cells treated with Noc as per a. Plot shows percentage of cells with indicated number of chromosomes lost or gained. Data are presented as mean？±？SD of two independent experiments. d Representative images of interphase FISH of RPE-1 cells treated with Noc as per a with probes for chromosome 6 (green) and chromosome 8 (red). Plot shows distribution of cell ploidy. ‘An’ represents nuclei with different numbers of signals for chromosomes 6 and 8. Scale bar, 10？μm. n？=？50 cells. e, f Representative metaphase spreads from (e) RPE-1 shp53 cells and (f) HCT116 p53-null cells treated with Rev as per b. Plots show percentage of cells with indicated number of chromosomes gained or lost. Chr denotes chromosome. Near-triploidy: 60–80 chromosomes; near-tetraploidy/tetraploidy: 81–110 chromosomes; near-octoploidy/octoploidy: 170–182 chromosomes. Scale bar, 20？μm. Minimum of 50 spreads were analysed per conditio