%0 Journal Article
%T Chromosomal instability-induced senescence potentiates cell non-autonomous tumourigenic effects
%A Bijin Au
%A Cheng. Kit. Wong
%A Elaine H. Lim
%A Giulia Rancati
%A Han C. Chong
%A Indrajit Sinha
%A Jiamila Maimaiti
%A John. E. Connolly
%A Kah. J. Lim
%A Karen C. Crasta
%A Madhura Kulkarni
%A Monique. N. H. Luijten
%A Qianqian He
%A Xiaomeng Wang
%A Yang Shen
%A Zhiyan Fu
%J Archive of "Oncogenesis".
%D 2018
%R 10.1038/s41389-018-0072-4
%X a, b Schematic representation of aneuploidy induction using either (a) 8 or 16£¿h nocodazole (Noc) or (b) 0.2 or 1£¿¦ÌM reversine (Rev). c Representative images of metaphase spreads from RPE-1 shp53 cells treated with Noc as per a. Plot shows percentage of cells with indicated number of chromosomes lost or gained. Data are presented as mean£¿¡À£¿SD of two independent experiments. d Representative images of interphase FISH of RPE-1 cells treated with Noc as per a with probes for chromosome 6 (green) and chromosome 8 (red). Plot shows distribution of cell ploidy. ¡®An¡¯ represents nuclei with different numbers of signals for chromosomes 6 and 8. Scale bar, 10£¿¦Ìm. n£¿=£¿50 cells. e, f Representative metaphase spreads from (e) RPE-1 shp53 cells and (f) HCT116 p53-null cells treated with Rev as per b. Plots show percentage of cells with indicated number of chromosomes gained or lost. Chr denotes chromosome. Near-triploidy: 60¨C80 chromosomes; near-tetraploidy/tetraploidy: 81¨C110 chromosomes; near-octoploidy/octoploidy: 170¨C182 chromosomes. Scale bar, 20£¿¦Ìm. Minimum of 50 spreads were analysed per conditio
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092349/