A dual role of miR-22 modulated by RelA/p65 in resensitizing fulvestrant-resistant breast cancer cells to fulvestrant by targeting FOXP1 and HDAC4 and constitutive acetylation of p53 at Lys382

a Total RNA isolated from the 182R-6 cell line and its parental S05 cell line was subjected to qRT-PCR analysis using a primer set for hsa-miR-22. RNU6-2 was used as a reference gene to normalize miR-22 expression. b Whole cellular lysates prepared from S05 and 182R-6 cells were subjected to western blot analysis using antibodies to either AGO2 or Dicer or Drosha; actin served as a loading control. c S05 and 182R-6 cells grown to 60–75% confluency on glass coverslips were fixed with 4% paraformaldehyde and permeabilized with ice-cold methanol. Immunofluorescence staining was performed using antibodies to either AGO2 or Dicer, as described in the “Methods” section. * indicates p？<？0.0