Using cell sheets to regenerate mouse submandibular glands

SMG cells form single sheets. a SMG tissue was dissociated using a GentleMACS and plated on a thermoresponsive culture dish at 37？°C for eight days, as described in Methods. Dish temperature was then reduced to 25？°C, which in turn caused the cells to detach from the surface and subsequently maintained intact the extracellular matrix proteins as compared to a traditional cell isolation method using trypsin, in which these proteins quickly disperse. b Sequence of cells detaching from the thermoresponsive plate depicted at 3, 5, and 8？min, with complete detachment occurring after 30？min. White bars represent 200？μm. c Single layer cell sheets were embedded in paraffin, sectioned, stained with H&E and imaged using a Leica DMI6000B inverted microscope at 10×. Black bars represent 100？μm. The diagram was drawn by author Kihoon Na