%0 Journal Article
%T Using cell sheets to regenerate mouse submandibular glands
%A Callie T. Brown
%A Kihoon Nam
%A Kyungsook Kim
%A Olga J. Baker
%A Ryan S. Davis
%A Spencer M. Dean
%A Teruo Okano
%J Archive of "NPJ Regenerative Medicine".
%D 2019
%R 10.1038/s41536-019-0078-3
%X SMG cells form single sheets. a SMG tissue was dissociated using a GentleMACS and plated on a thermoresponsive culture dish at 37£¿¡ãC for eight days, as described in Methods. Dish temperature was then reduced to 25£¿¡ãC, which in turn caused the cells to detach from the surface and subsequently maintained intact the extracellular matrix proteins as compared to a traditional cell isolation method using trypsin, in which these proteins quickly disperse. b Sequence of cells detaching from the thermoresponsive plate depicted at 3, 5, and 8£¿min, with complete detachment occurring after 30£¿min. White bars represent 200£¿¦Ìm. c Single layer cell sheets were embedded in paraffin, sectioned, stained with H&E and imaged using a Leica DMI6000B inverted microscope at 10¡Á. Black bars represent 100£¿¦Ìm. The diagram was drawn by author Kihoon Na
%K Regenerative medicine
%K Preclinical research
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609686/