Regulation of exocytosis and mitochondrial relocalization by Alpha-synuclein in a mammalian cell model

Structural features of a-syn. a Schematic representation of the a-syn primary sequence delineating the amphipathic membrane-binding domain (orange) and the acidic C-terminal tail (green), and indicating the locations of the NAC domain, helix-1 and helix-2 of the broken-helix state, and the sites of PD and other mutations examined in the manuscript. b Cartoon model of Wt a-syn in an extended helix conformation. The model was generated from RCSB protein data bank entry 1XQ8 by manually converting the non-helical linker between helix-1 and helix-2 to a helical conformation. The sidechains of residues Ala 30 and Val 70 are shown to indicate the location of proline mutations examined in this study. c Wt a-syn in the broken helix conformation (RCSB protein data bank entry 1XQ8) with the sidechains of Ala 30 and Val 70 show