Peptidyl-prolyl isomerase-B is involved in Mycobacterium tuberculosis biofilm formation and a generic target for drug repurposing-based intervention

M.tb PpiB protein enhances biofilm and pellicle formation in recombinant M.smegmatis: M. tb PpiA and PpiB genes were cloned in M.smegmatis, as described in methods, to generate Ms_PpiA and Ms_PpiB strains, respectively. M.smegmatis vector control (Ms_VC), Ms_PpiA and Ms_PpiB were grown in static culture and induced with anhydrotetracycline for expressing M.tb PPIases. a Pellicle formed in Ms_VC, Ms_PpiA and Ms_PpiB cultures from a representative experiment. The pellicle formed at the liquid air interface was quantified as described in methods. Values shown in b are mean [±s.e.m] from three independent experiments and depict the amount of biofilm formed in Ms_VC, Ms_PpiA and Ms_PpiB cultures. ***p？<？0.005 vs. Ms_VC (Student’s t-test