%0 Journal Article
%T Peptidyl-prolyl isomerase-B is involved in Mycobacterium tuberculosis biofilm formation and a generic target for drug repurposing-based intervention
%A Aditi Singh
%A Anwar Alam
%A Ashutosh Kumar
%A Deeksha Tripathi
%A Mamta Rani
%A Monika Kumari
%A Nasreen Z. Ehtesham
%A Saurabh Pandey
%A Seyed E. Hasnain
%A Sonam Grover
%A Yusuf Akhter
%J Archive of "NPJ Biofilms and Microbiomes".
%D 2019
%R 10.1038/s41522-018-0075-0
%X M.tb PpiB protein enhances biofilm and pellicle formation in recombinant M.smegmatis: M. tb PpiA and PpiB genes were cloned in M.smegmatis, as described in methods, to generate Ms_PpiA and Ms_PpiB strains, respectively. M.smegmatis vector control (Ms_VC), Ms_PpiA and Ms_PpiB were grown in static culture and induced with anhydrotetracycline for expressing M.tb PPIases. a Pellicle formed in Ms_VC, Ms_PpiA and Ms_PpiB cultures from a representative experiment. The pellicle formed at the liquid air interface was quantified as described in methods. Values shown in b are mean [¡Às.e.m] from three independent experiments and depict the amount of biofilm formed in Ms_VC, Ms_PpiA and Ms_PpiB cultures. ***p£¿<£¿0.005 vs. Ms_VC (Student¡¯s t-test
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333787/