Blocking CXCLs–CXCR2 axis in tumor–stromal interactions contributes to survival in a mouse model of pancreatic ductal adenocarcinoma through reduced cell invasion/migration and a shift of immune-inflammatory microenvironment

a Top 10 upregulated genes in CAFs after stimulation with conditioned medium (CM) of PDAC cell lines derived from PKF (Ptf1acre/+;LSL-KrasG12D/+;Tgfbr2flox/flox;Cxcr2+/+) mice in the microarray analysis. Cxcl genes are red-colored. b qRT-PCR analysis in CAFs (311f, 545f) after addition of CM from mPanIN cells (PK-CM) or PDAC cells (PKF-CM). Data are means？±？standard error (SE). **p？<？0.01 compared to the control (C) and (PK-CM). c Invasive activity of PDAC with CM of CAFs with/without CXCR2 inhibitor SB225002. Data are means？±？SE. *p？<？0.05 compared to the CAF-CM. d Migration of CAFs with CM of mPanIN or PDAC. Data are means？±？SE. *p？<？0.05, **p？<？0.01 compared to the control. e Inhibition of CAF migration by CXCR2 inhibitor SB225002. Data are means？±？SE. **p？<？0.01 compared to the PDAC-C