Metabolic reprogramming underlies metastatic potential in an obesity-responsive murine model of metastatic triple negative breast cancer

Generation and in vitro characterization of metM-Wntlung cells. a Representative IVIS image of C57BL/6 mice injected via tail vein with GFP-Luciferase expressing M-Wnt cells, showing no resulting metastasis. b Representative IVIS image of SCID mice injected with GFP-Luciferase expressing M-Wnt tumor brei, following survival surgery to remove tumor and resultant metastases in lung (left to right). c Colony formation assay of E-Wnt, M-Wnt, and metM-Wntlung cells and d colony formation in soft agarose. e Matrigel invasion assay and f adhesion assay of E-Wnt, M-Wnt and metM-Wntlung cells. g Representative flow cytometry analysis of E-Wnt, M-Wnt and metM-Wntlung cells stained with antibodies specific for CD44 (y-axis) and CD24 (x-axis), quantified in right-hand graph. All in vitro experiments are inclusive of three independent experiments. *p？<？0.05, **p？<？0.01, ***p？<？0.001, ns non-significant, as compared with M-Wn