%0 Journal Article
%T Metabolic reprogramming underlies metastatic potential in an obesity-responsive murine model of metastatic triple negative breast cancer
%A Charles M. Perou
%A Ciara H. O¡¯Flanagan
%A Emily L. Rossi
%A Jerry Usary
%A Joel S. Parker
%A Shannon B. McDonell
%A Stephen D. Hursting
%A Xuewen Chen
%A Yi-Hsuan Tsai
%J Archive of "NPJ Breast Cancer".
%D 2017
%R 10.1038/s41523-017-0027-5
%X Generation and in vitro characterization of metM-Wntlung cells. a Representative IVIS image of C57BL/6 mice injected via tail vein with GFP-Luciferase expressing M-Wnt cells, showing no resulting metastasis. b Representative IVIS image of SCID mice injected with GFP-Luciferase expressing M-Wnt tumor brei, following survival surgery to remove tumor and resultant metastases in lung (left to right). c Colony formation assay of E-Wnt, M-Wnt, and metM-Wntlung cells and d colony formation in soft agarose. e Matrigel invasion assay and f adhesion assay of E-Wnt, M-Wnt and metM-Wntlung cells. g Representative flow cytometry analysis of E-Wnt, M-Wnt and metM-Wntlung cells stained with antibodies specific for CD44 (y-axis) and CD24 (x-axis), quantified in right-hand graph. All in vitro experiments are inclusive of three independent experiments. *p£¿<£¿0.05, **p£¿<£¿0.01, ***p£¿<£¿0.001, ns non-significant, as compared with M-Wn
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514148/