Familial Parkinson’s Disease-Associated L166P Mutant DJ-1 is Cleaved by Mitochondrial Serine Protease Omi/HtrA2

Omi is involved in the instability of L166P. A Immunoblots of lysates of N2a cells transfected with equal amounts of Flag-DJ-1 or Flag-L166P construct for 36 h and using the indicated antibodies. B Ratios of Flag-DJ-1 or Flag-L166P relative to α-tubulin from (A) from densitometric analysis Data are from three independent experiments (mean ± SEM; *P < 0.05; ns, no statistical significance, one-way ANOVA). C Immunoblots of lysates of H1299 cells co-transfected with Flag-DJ-1 or Flag-L166P and HA, HA-Omi or HA-S276C for 36 h as indicated. D Ratios of Flag-DJ-1 or Flag-L166P relative to α-tubulin from (C) from densitometric analysis. Data from three independent experiments (mean ± SEM, *P < 0.05; ns, no statistical significance, one-way ANOVA