Upregulation of B7-H4 promotes tumor progression of intrahepatic cholangiocarcinoma

High expression of B7-H4 in tumor tissues of ICC patients. a qRT-PCR showed that the expression of B7-H4 mRNA in tumor tissues was higher than that in correspondingly peritumoral tissues (*p？<？0.05). b Representative image showed expression of B7-H4 protein in tumor samples and peritumoral tissues from 35 ICC patients by western blotting. c Semi-quantitative analysis showed that the expression of B7-H4 protein in tumors tissues was higher than that in peritumoral tissues(*p？<？0.05). d Representative image showed the expression of B7-H4 protein in tumor samples and peritumoral tissues from 140 ICC patients by immunohistochemical staining. e Semi-quantitaive analysis for IHC staining showed that B7-H4 protein expression in tumor samples was higher than that in peritumoral tissues (**p？<？0.01). f The expression of B7-H4 in tumor tissues from ICC patients with early recurrence after operation was higher than that in patients without recurrence (*p？<？0.05). g qRT-PCR from 35 ICC patients showed that the expression of B7-H4 mRNA in tumor tissues of ICC patients with early recurrence was much higher than that of patients without recurrence (***p？<？0.01). h Representative image showed expression of B7-H4 protein in 35 randomly selected ICC pateints by western blotting. i Semi-quantitaive analysis for western blotting showed that the expression of B7-H4 in tumor tissues from ICC patients with early recurrence was higher than that in patients without recurrence (**p？<？0.01