MiR-873-5p acts as an epigenetic regulator in early stages of liver fibrosis and cirrhosis

(a) Luciferase reporter assay of Gnmt 3？UTR expression in hepatocytes transfected with mimic-miR-873-5p (N？=？8). (b) GNMT and miR-873-5p expression at mRNA and protein levels in primary hepatocyte after mimic-miR-873-5p transfection. Gnmt-miR-873-5p expression and correlation and WB analysis of GNMT at different time points of BDL (N？=？3) (c) and in Mdr2WT and Mdr2-/- mice at different ages (3,4,9,12,17 months each column and 3▲, 4▼, 9？, 12●, and 17■ months in the correlation panel) (N？=？3) (d). Densitometry analyses of WB are shown in Supp. Fig. 1B, D, E. Data normalized as fold change vs. control. Error bars represent the means？±？SEM. Statistical significance was determined by the Student’s t-test or ANOVA when more than two groups were compared. p？<？0.05*; p？<？0.01**; p？<？0.001**