Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer

Exogenous FOXP3 interacts with Gal-1. HEK293T cells were transfected with Flag-tagged FOXP3 (or empty vector) and Gal-1 (or empty vector). Co-IP experiment was performed using (a) anti-Gal-1 antibody or (b) anti-Flag antibody. The immunoprecipitates were analyzed via western blotting with anti-Flag or anti-Gal-1 antibody. c Endogenous FOXP3 interacts with Gal-1. Co-IP experiment was performed using anti-FOXP3 antibody in MCF-7 and T47D cells. IgG was used as the negative control. The immunoprecipitates were analyzed via western blotting with anti-FOXP3 and anti-Gal-1 antibodies. d T47D cells were transfected with FOXP3-eGFP and Gal-1-dsRed. FRET microscopy was used to observe the localization of the fusion proteins in these cells. Scale bar？=？20？μm. e Cells in d were analyzed for FRET signals. The color bar represents FRET efficiency (purple indicates the absence of FRET signal). f Visualization of the FOXP3/Gal-1 complex. The overall structure is shown as a cartoon, where each protein is colored differently. FOXP3 is shown in green, and Gal-1 is shown in blu