%0 Journal Article
%T Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer
%A Cheng Zeng
%A Cun Zhang
%A Dong Fan
%A Huadong Zhao
%A Kuo Zhang
%A Meng Li
%A Qiang Hao
%A Shuning Wang
%A Wangqian Zhang
%A Wei Zhang
%A Weina Li
%A Xiaochang Xue
%A Xiaoju Li
%A Yingqi Zhang
%A Yuan Gao
%A Zhaowei Wang
%A Zhen Shu
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-018-0448-6
%X Exogenous FOXP3 interacts with Gal-1. HEK293T cells were transfected with Flag-tagged FOXP3 (or empty vector) and Gal-1 (or empty vector). Co-IP experiment was performed using (a) anti-Gal-1 antibody or (b) anti-Flag antibody. The immunoprecipitates were analyzed via western blotting with anti-Flag or anti-Gal-1 antibody. c Endogenous FOXP3 interacts with Gal-1. Co-IP experiment was performed using anti-FOXP3 antibody in MCF-7 and T47D cells. IgG was used as the negative control. The immunoprecipitates were analyzed via western blotting with anti-FOXP3 and anti-Gal-1 antibodies. d T47D cells were transfected with FOXP3-eGFP and Gal-1-dsRed. FRET microscopy was used to observe the localization of the fusion proteins in these cells. Scale bar£¿=£¿20£¿¦̀m. e Cells in d were analyzed for FRET signals. The color bar represents FRET efficiency (purple indicates the absence of FRET signal). f Visualization of the FOXP3/Gal-1 complex. The overall structure is shown as a cartoon, where each protein is colored differently. FOXP3 is shown in green, and Gal-1 is shown in blu
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856744/