RB1CC1-enhanced autophagy facilitates PSCs activation and pancreatic fibrogenesis in chronic pancreatitis

a, b The heatmap and volcano plots showed that 433 genes were changed in activated PSCs compared with quiescent PSCs (fold change ≥2 or ≤0.5, p？≤？0.05). c Top ten upregulated genes were listed and RB1CC1 was significantly elevated. d, e The expressions of RB1CC1 protein and mRNA were explored in quiescent and activated PSCs (Bars？=？500μm). f Western blot analyses were detected to determine the expressions of RB1CC1, α-SMA, Fibronectin, Collagen I, and Collagen III in quiescent and TGF-β-treated PSCs. GAPDH served as the internal control. g, h Gene classification and gene function enrichment analysis of differentially expressed genes were performed (*p？<？0.05 and **p？<？0.01