Antidiabetic adiponectin receptor agonist AdipoRon suppresses tumour growth of pancreatic cancer by inducing RIPK1/ERK-dependent necroptosis

a, b Cell cycle progression of MIAPaCa-2 cells. The cells treated with solvent (DMSO) alone, 50？μM and 100？μM AdipoRon for 24？h were subjected to flow cytometric analysis. c Survival of MIAPaCa-2 cells and HPAEpiC cells. The cells were treated with various concentrations of AdipoRon for 40？h. d Survival of human pancreatic cancer cell lines. The cells were treated with various concentrations of AdipoRon for 6 days. e qRT-PCR analysis of the knockdown of AdipoR1 and AdipoR2 expression. f Effect of the knockdown of AdipoR1 and AdipoR2 expression on the survival of MIAPaCa-2 cells treated with solvent alone (V) or 100？μM AdipoRon for 40？h. For c–f, error bars represent standard deviation. *P？<？0.05, **P？<？0.001 by ANOVA test. ns not significan