Synergistic effect of a novel autophagy inhibitor and Quizartinib enhances cancer cell death

a Outline of high-throughput screen of cytotoxicity of 12,640 compounds with or without AC220 in ES-2 cells. b Left panel: Fold cell survival data for the screened compounds with (y-axis) or without (x-axis) AC220, with respect to untreated control. Right panel: Student’s t test with respect to untreated control showing the most statistically significant hits. TAK-165 was discovered among the top 45 hits that did not induce cell death alone, but induced cell death in combination with AC220. c TAK-165 (Mubritinib) chemical structure. d Dose-response curve of TAK-165 alone and in combination with AC220 in ES-2 cells. ES-2 cells were treated with TAK-165 at indicated concentrations and AC220 at 2？μM for 24？h. Viability was determined using CellTiter-Glo？ Luminescent assay (n？=？3). Bars: Mean？±？SD. *p？<？0.05; **p？<？0.01; ***p？<？0.00