Aberrant NFATc1 signaling counteracts TGFβ-mediated growth arrest and apoptosis induction in pancreatic cancer progression

a Hematoxylin and eosin (HE) and immunohistochemical of NKC p48 mice representing different age groups corresponding to different stages of PDAC development (×400, scale bar: 100？μm). b HE stainings (×200, scale bar: 100？μm) certify established PDAC of three individual NKC p48 mice. Western blot analysis of the indicated proteins upon TGFβ treatment (10？ng/ml) in primary PDAC cells derived from respective NKC p48 mice (NKC cells). c Immunofluorescence demonstrating TGFβ-mediated translocation of receptor Smads (Smad2/3) into nuclei of primary PDAC cells (×400, scale bar: 25？μm). d Wound healing assay (n？=？4) and e invasion assay in the presence and absence of TGFβ (n？=？3