Loss of MADD expression inhibits cellular growth and metastasis in anaplastic thyroid cancer

MADD gene silencing reduces ATC growth in vitro and in vivo: a Quantitative RT-PCR showed significantly higher expression of MADD mRNA in 8505？C, C643 and HTH7 cells in comparison to the normal human thyroid tissues. b MADD siRNA treatment resulted in a significant reduction in MADD protein expression, depicted by Western blotting. β-actin was used to normalize the expression levels. c MTT assay exhibited a significant reduction in proliferation MADD depleted cells after 72？h in 8505？C, C643 and HTH7 cells. d Representative photograph of 8505？C orthotopic tumor-bearing mice treated with MADD siRNA in comparison to the untreated control and scramble siRNA-treated mice. e, f Intra-tumoral treatment with MADD siRNA showed a significant reduction in the tumor volume and weight, as compared to the untreated control and scramble siRNA-treated tumors. All the values represent the mean？±？SD of triplicate samples from a typical experiment. *P？<？0.05, **P？<？0.005, and ***P？<？0.0005, by the two-tailed student’s t tes