%0 Journal Article
%T Loss of MADD expression inhibits cellular growth and metastasis in anaplastic thyroid cancer
%A Ajay V. Maker
%A Bellur S. Prabhakar
%A Fei Yue
%A Kiara Tulla
%A Lakshmi Sripada
%A Nicholas Kunda
%A Prabhakaran Kumar
%A Shikha Saini
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1351-5
%X MADD gene silencing reduces ATC growth in vitro and in vivo: a Quantitative RT-PCR showed significantly higher expression of MADD mRNA in 8505£¿C, C643 and HTH7 cells in comparison to the normal human thyroid tissues. b MADD siRNA treatment resulted in a significant reduction in MADD protein expression, depicted by Western blotting. ¦Â-actin was used to normalize the expression levels. c MTT assay exhibited a significant reduction in proliferation MADD depleted cells after 72£¿h in 8505£¿C, C643 and HTH7 cells. d Representative photograph of 8505£¿C orthotopic tumor-bearing mice treated with MADD siRNA in comparison to the untreated control and scramble siRNA-treated mice. e, f Intra-tumoral treatment with MADD siRNA showed a significant reduction in the tumor volume and weight, as compared to the untreated control and scramble siRNA-treated tumors. All the values represent the mean£¿¡À£¿SD of triplicate samples from a typical experiment. *P£¿<£¿0.05, **P£¿<£¿0.005, and ***P£¿<£¿0.0005, by the two-tailed student¡¯s t tes
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374448/