Blockade of CTLA-4 and Tim-3 pathways induces fetal loss with altered cytokine profiles by decidual CD4+T cells

a The weight of pregnant CBA/J females treated with isotype IgG, anti-CTLA-4, anti-Tim-3 antibody, both antibodies i.p. at doses of 500, 250, and 250？mg at days 4.5, 6.5, and 8.5, respectively. b The number of live fetuses per uterus from pregnant CBA/J females following treatment with the indicated blocking antibodies. c–e Quantification of flow cytometric analysis of cytokines production and transcription factors expression by dCD4+T cells from pregnant CBA/J female mice following treatment with the indicated blocking antibodies. Data represented the mean？±？standard error of the mean (SEM) of n？=？6–10mice per group and were representative of four independent analyses. *P？<？0.05, **P？<？0.01, ***P？<？0.001, compared with the control group. #P？<？0.05, ##P？<？0.01, ###P？<？0.001, compared with the group of anti-CTLA-4 and anti-Tim-