Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development

a Schematic representation of the experimental procedures using CCl4-induced liver fibrosis followed by BMT in a mouse model. b Flow cytometric analysis for the expression of CD25 and CD44 on thymocyte for DN1–DN4 stages of the T-cell development in the thymus on day 28 after CD45.1 BMT through the hepatic portal vein. c Flow cytometric analysis for the expression of CD4 and CD8 in thymocytes for the DP and SP stages of T-cell development, on day 28 after CD45.1 BMT. d Flow cytometric analysis for the expression of CD4 and CD8 on PBMCs for CD4+ and CD8+ T cells in peripheral blood on day 28 after CD45.1 BMT. The results are presented as mean？±？S.E.M. Statistical significance was determined by Student’s t test. Significance between samples is indicated in the figures as follows: *P？<？0.05; **P？<？0.0