c-kit Haploinsufficiency impairs adult cardiac stem cell growth, myogenicity and myocardial regeneration

a The fraction of necrotic cardiomyocytes (CM) was significantly increased in a dose-dependent manner at 1 day after ISO (200 and 400？mg/Kg) with higher frequency in the Apex sub-endocardium. n？=？7 per group; *p？<？0.05 vs. saline; #p？<？0.05 vs. Epi; ？p？<？0.05 vs. Mid; ？p？<？0.05 vs. 200？mg/Kg (one-way ANOVA analysis with Tukey’s multiple comparison test). b Representative images of necrotic CMs labelled in vivo with anti-myosin antibody (green) showing higher damage in the sub-endocardium when compared with myocardium and epicardium. c, d Representative images of necrotic CMs labelled in vivo with anti-myosin antibody (green) showing ISO dose-dependent higher damage in the apex when compared with mid and base in LV regions 1 day after ISO 200 (c) or 400 (d) mg/Kg, respectively. e Representative image of a non-necrotic anti-myosin antibody (green)-labelled normal cardiomyocyte. f Plasma cTnI was significantly elevated in a dose-dependent manner at 1 day after ISO. hs-TnT？=？high-sensitive cardiac Troponin T. n？=？7 per group; *P？<？0.05 vs. Saline; #p？<？0.05 vs. 200？mg/Kg (one-way ANOVA analysis with Tukey’s multiple comparison test). Scale bars？=？50？μm except for b left panel？=？300？μm. All data are mean？±？S