%0 Journal Article
%T c-kit Haploinsufficiency impairs adult cardiac stem cell growth, myogenicity and myocardial regeneration
%A Andrea Filardo
%A Andrea M. Isidori
%A Andrew J. Smith
%A Antonella De Angelis
%A Bernardo Nadal-Ginard
%A Daniele Torella
%A Donato Cappetta
%A Eleonora Cianflone
%A Fabiola Marino
%A Georgina M. Ellison-Hughes
%A Giuseppe Viglietto
%A Iolanda Aquila
%A Konrad Urbanek
%A Mariangela Scalise
%A Michele Torella
%A Teresa Mancuso
%A Valter Agosti
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1655-5
%X a The fraction of necrotic cardiomyocytes (CM) was significantly increased in a dose-dependent manner at 1 day after ISO (200 and 400£¿mg/Kg) with higher frequency in the Apex sub-endocardium. n£¿=£¿7 per group; *p£¿<£¿0.05 vs. saline; #p£¿<£¿0.05 vs. Epi; £¿p£¿<£¿0.05 vs. Mid; £¿p£¿<£¿0.05 vs. 200£¿mg/Kg (one-way ANOVA analysis with Tukey¡¯s multiple comparison test). b Representative images of necrotic CMs labelled in vivo with anti-myosin antibody (green) showing higher damage in the sub-endocardium when compared with myocardium and epicardium. c, d Representative images of necrotic CMs labelled in vivo with anti-myosin antibody (green) showing ISO dose-dependent higher damage in the apex when compared with mid and base in LV regions 1 day after ISO 200 (c) or 400 (d) mg/Kg, respectively. e Representative image of a non-necrotic anti-myosin antibody (green)-labelled normal cardiomyocyte. f Plasma cTnI was significantly elevated in a dose-dependent manner at 1 day after ISO. hs-TnT£¿=£¿high-sensitive cardiac Troponin T. n£¿=£¿7 per group; *P£¿<£¿0.05 vs. Saline; #p£¿<£¿0.05 vs. 200£¿mg/Kg (one-way ANOVA analysis with Tukey¡¯s multiple comparison test). Scale bars£¿=£¿50£¿¦Ìm except for b left panel£¿=£¿300£¿¦Ìm. All data are mean£¿¡À£¿S
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547756/