Dissecting cell diversity and connectivity in skeletal muscle for myogenesis

a Workflow of the protocol to generate skeletal muscle organoid from seed cells and matrigel via myogenic cultured in differentiation medium (DM). b Images of skeletal organoid under camera during formation after 14 days culture, R: RACs as seed cells cultured alone, MR: MPCs and RACs co-cultured, M: MPCs as seed cells cultured alone, Scale bars, 1？mm. c Images of skeletal organoid under ×40 microscope during formation after 14 days culture, Scale bars, 100？μm. d Representative images of myosin heavy chain (MHC) immunofluorescence staining in skeletal muscle organoid three groups: R, MR, M. e Ultrastructure of skeletal muscle organoid at 2 weeks of culturing. From left to right: Transmitted electron microscopy images of MPCs and RACs co-cultured in 3D culturing environment for 2 weeks (yellow arrowhead: z lines); Transmitted electron microscopy images of RACs cultured in 3D culturing environment for 2 weeks; Transmitted electron microscopy images of MPCs cultured in 3D culturing environment for 2 weeks (yellow arrowhead: z lines