%0 Journal Article
%T Dissecting cell diversity and connectivity in skeletal muscle for myogenesis
%A Asma Mechakra
%A Bing-bing Wu
%A Cheng-rui An
%A De-ming Jiang
%A Hong-Wei Ouyang
%A Jun-xin Lin
%A Kai-xiu Jin
%A Lin Gong
%A Qi-kai Li
%A Varitsara Bunpetch
%A Xiao-Hui Zou
%A Yi-wei Zou
%A Yi-xiao Liu
%A Yu Li
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1647-5
%X a Workflow of the protocol to generate skeletal muscle organoid from seed cells and matrigel via myogenic cultured in differentiation medium (DM). b Images of skeletal organoid under camera during formation after 14 days culture, R: RACs as seed cells cultured alone, MR: MPCs and RACs co-cultured, M: MPCs as seed cells cultured alone, Scale bars, 1£¿mm. c Images of skeletal organoid under ¡Á40 microscope during formation after 14 days culture, Scale bars, 100£¿¦Ìm. d Representative images of myosin heavy chain (MHC) immunofluorescence staining in skeletal muscle organoid three groups: R, MR, M. e Ultrastructure of skeletal muscle organoid at 2 weeks of culturing. From left to right: Transmitted electron microscopy images of MPCs and RACs co-cultured in 3D culturing environment for 2 weeks (yellow arrowhead: z lines); Transmitted electron microscopy images of RACs cultured in 3D culturing environment for 2 weeks; Transmitted electron microscopy images of MPCs cultured in 3D culturing environment for 2 weeks (yellow arrowhead: z lines
%K Extracellular signalling molecules
%K Muscle stem cells
%K Muscle stem cells
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546706/