CUL1 promotes breast cancer metastasis through regulating EZH2-induced the autocrine expression of the cytokines CXCL8 and IL11

a The CUL1 protein expression in human normal human mammary epithelial cell line MCF10A and breast cancer cell lines were determined by western blotting. b MCF10A cells were transiently transfected with CUL1 overexpression (PCDNA3.1-CUL1) and vector control (PCDNA3.1) plasmids. c The migration and invasion of MCF10A cells with CUL1 overexpression and vector control. d The number of cell migration and invasion per field was counted in five random fields (n？=？3/group). e Western blots of EMT-related markers E-cadherin, β-catenin, N-cadherin, Vimentin, and Fibronectin in MCF10A with CUL1 overexpression and vector control. f Relative mRNA expression levels of EMT markers (normalization to GAPDH) in MCF10A cells with CUL1 overexpression and vector control. Data are presented as means？±？standard deviations. *P？<？0.05, **P？<？0.001 (Student’s t-test