Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement

Effect of Eg5 inhibition on the porcine oocyte maturation. A Representative images of control and Eg5-inhibited oocytes cultured in vitro for 44 h. Cumulus cell expansion of COCs and polar body extrusion of DOs were imaged by confocal microscope. Scale bar, 150 μm (a, b); 80 μm (c, d); 20 μm (e, f). B The rates of polar body extrusion were recorded in control and different concentrations (10, 50 and 100 μM) of monastrol-treated oocytes. C The percentage of control and Eg5-inhibited oocytes in GV, M I or M II stage after in vitro maturation. D Oocytes that underwent GVBD were recorded in control and monastrol-treated groups. Scale bar, 5 μm. Data of B–D were presented as mean percentage (mean？±？SEM) of at least three independent experiments. **p？<？0.0