%0 Journal Article
%T Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement
%A Bo Xiong
%A Chen Pan
%A Dongyu Yang
%A Minghui Cheng
%A Qilong Yuan
%A Shan Lu
%A Yan Xie
%A Ying Chen
%A Yurong Qiu
%J Archive of "Cell Division".
%D 2018
%R 10.1186/s13008-018-0037-1
%X Effect of Eg5 inhibition on the porcine oocyte maturation. A Representative images of control and Eg5-inhibited oocytes cultured in vitro for 44 h. Cumulus cell expansion of COCs and polar body extrusion of DOs were imaged by confocal microscope. Scale bar, 150 ¦Ìm (a, b); 80 ¦Ìm (c, d); 20 ¦Ìm (e, f). B The rates of polar body extrusion were recorded in control and different concentrations (10, 50 and 100 ¦ÌM) of monastrol-treated oocytes. C The percentage of control and Eg5-inhibited oocytes in GV, M I or M II stage after in vitro maturation. D Oocytes that underwent GVBD were recorded in control and monastrol-treated groups. Scale bar, 5 ¦Ìm. Data of B¨CD were presented as mean percentage (mean£¿¡À£¿SEM) of at least three independent experiments. **p£¿<£¿0.0
%K Oocyte meiotic maturation
%K Spindle assembly
%K Actin dynamics
%K Mitochondrion integrity
%K Cortical granule
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966870/