Extracorporeal photochemotherapy induces bona fide immunogenic cell death

a Residual number of B16-OVA cells upon exposure to 8-methoxypsoralen (8-MOP) in the context of UVA light at the indicated dose, 4J UVA irradiation only, or the indicated dose of cisplatin (CDDP) for 48？h, or upon exposure to repeated freeze/thawing (F/T) and culture in control conditions for 48？h. Representative micrographs and quantitative data are reported. n？=？3; **p？<？0.01, ***p？<？0.001 (Student’s t-test), as compared to cells receiving 4J UVA light only. b Percentage of AnnV+PI？ and AnnV+PI+ B16-OVA cells upon exposure to 8-MOP plus 4J UVA light (8-MOPA) and culture in control conditions for the indicated time. Representative dotplots with percentages of events in relevant quadrants, and quantitative results are reported. n？=？3; *p？<？0.05, ***p？<？0.001 (Student’s t-test), as compared to untreated cells. c Residual clonogenic potential of B16-OVA cells optionally exposed to 8-MOP in the context of no irradiation or UVA light at the indicated dose. Representative images and quantitative results are reported. n？=？3; ***p？<？0.001 (Student’s t-test), as compared to cells exposed to the same UVA dose in the absence of 8-MOP. d Percentage of C57BL/6 mice developing tumors upon subcutaneous inoculation of B16-OVA cells exposed in vitro to the indicated UVA dose in the presence or absence of 8-MOP. Quantitative results from one representative experiment involving 10 mice per group are reporte